Screening Technique Based on Seed and Early Seedling Parameters for Cold Tolerance of Selected F2-Derived F3 Rice Genotypes under Controlled Conditions

The cold tolerance studies were carried out in a bi-parental F2 population of cross between tolerant and susceptible parents (SKUA-529 HEERA, respectively). purpose was to screen the individuals for primary cold-tolerance-related attributes. information generated has direct application use identifying quantitative trait loci (QTLs) further can be used genotyping with an appropriate marker system. screening on F2-derived F3 seeds plants seedling agronomic traits, respectively. Two tests measuring conducted. In experiment I, germinated 28 days at 13 °C 7 °C, II, 72 h 96 once more °C. Coleoptile length, germination percentage, radical reduction percentages all measured I. radicle coleoptile regeneration II after period. improvement achieved through regrowth, as evidenced by difference second first measurements. individual lines from F2:3 that recorded high (%) #21, #13, #14, #15. percentage length (PERCOL %) observed ranges 23.33% 53.00%. (REDCOL also obtained, there less #15, #16, #14 it had range 38.46% 75%. Radicle regrowth (REDRAG) #7, #11, #30, #35, #36. Survival 33.33% up 100%, highest survival rate #16. main objective this rotation temperature emulate field conditions where been drop temperature. evaluations done stress found most these traits exhibited variability. mapping developed may utilized generate linkage map locate QTLs rice. Further, identified donors breeding programs aimed transfer low-temperature into backgrounds. general, genotype improved rates, growth leaf yellowing scores; survival; lesser development; recovery following shock stage demonstrated its resistance. Genotypes low greater number germination, slow higher score, growth, reduced indicated susceptibility.